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This is followed by continuous cell proliferation resulting in the formation of the root nodule. The bacteria then infect several other adjacent root cells. Nod factors trigger a series of complex developmental changes inside the root hair, beginning with root hair curling and followed by the formation of the infection thread, a cellulose lined tube that the bacteria use to travel down through the root hair into the root cells. These flavonoids then promote the DNA binding activity of NodD which belongs to the LysR family of transcriptional regulators and triggers the secretion of nod factors after the bacteria have entered the root hair. Rhizobia are free living in the soil until they are able to sense flavonoids, derivatives of 2-phenyl-1.4-benzopyrone, which are secreted by the roots of their host plant triggering the accumulation of a large population of cells and eventually attachment to root hairs. The most well understood mechanism for the establishment of this symbiosis is through intracellular infection. The formation of the symbiotic relationship involves a signal exchange between both partners that leads to mutual recognition and development of symbiotic structures. Given the costs involved in nodulation and the opportunity for rhizobia to cheat, it may be surprising that this symbiosis should exist at all. Cheater strains may hoard plant resources such as polyhydroxybutyrate for the benefit of their own reproduction without fixing an appreciable amount of nitrogen. However, because several unrelated strains infect each individual plant, a classic tragedy of the commons scenario presents itself. The legume–rhizobium symbiosis is a classic example of mutualism-rhizobia supply ammonia or amino acids to the plant and in return receive organic acids (principally as the dicarboxylic acids malate and succinate) as a carbon and energy source. Common crop and forage legumes are peas, beans, clover, and soy. Rhizobia are unique in that they are the only nitrogen-fixing bacteria living in a symbiotic relationship with legumes. As a result of the nodulation process, after the harvest of the crop there are higher levels of soil nitrate, which can then be used by the next crop. These inoculants may foster success in legume cultivation. An ideal inoculant includes some of the following aspects maximum efficacy, ease of use, compatibility, high rhizobial concentration, long shelf-life, usefulness under varying field conditions, and survivability. The technology to produce these inoculants are microbial fermenters. 12–20 million hectares of soybeans are inoculated annually. Legume inoculation has been an agriculture practice for many years and has continuously improved over time. Inoculation with rhizobia tends to increase yield. Having this specific rhizobia present is beneficial to the legume, as the N 2 fixation can increase crop yield. Specific strains of rhizobia are required to make functional nodules on the roots able to fix the N 2. Supply of nitrogen through fertilizers has severe environmental concerns. Nitrogen is the most commonly deficient nutrient in many soils around the world and it is the most commonly supplied plant nutrient. This is especially important when nitrogen fertilizer is not used, as in organic rotation schemes or some less- industrialized countries. Importance in agriculture Īlthough much of the nitrogen is removed when protein-rich grain or hay is harvested, significant amounts can remain in the soil for future crops. For instance, the plant pathogen Agrobacterium is a closer relative of Rhizobium than the Bradyrhizobium that nodulate soybean. These groups include a variety of non- symbiotic bacteria. Β-proteobacteria Burkholderiales Burkholderiaceae Cupriavidus C. terangae Xanthobacteraceae Azorhizobium A. neptuniae Methylobacteriaceae Methylobacterium M. yuanmingense Brucellaceae Ochrobactrum O. Α-proteobacteria Hyphomicrobiales Nitrobacteraceae Bosea Bradyrhizobium B. As shown below, most belong to the order Hyphomicrobiales, but several rhizobia occur in distinct bacterial orders of the proteobacteria. Rhizobia are a paraphyletic group that fall into two classes of proteobacteria-the alphaproteobacteria and betaproteobacteria. Most research has been done on crop and forage legumes such as clover, alfalfa, beans, peas, and soybeans more research is being done on North American legumes. The first known species of rhizobia, Rhizobium leguminosarum, was identified in 1889, and all further species were initially placed in the Rhizobium genus.
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